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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Nutr Biochem. 2011 Dec 1;23(9):1134–1145. doi: 10.1016/j.jnutbio.2011.06.007

Figure 5.

Figure 5

EGCG-stimulated increases in HO-1 protein expression did not require MEK, PI3K, or NOS activity. HAEC were serum-starved for 1 h and then pre-treated without or with various pathway-selective chemical inhibitors for 1 h (PD = PD98059, 25 μM; LY = LY294002, 50 μM; L-NAME, 100 μM). This was followed by treatment without or with EGCG (2.5 μM, 6 h). Cell lysates were immunoblotted using antibodies against HO-1, HO-2, and β-actin as indicated. Immunoblots shown are representative results from at least 3 independent experiments.