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Biophysical formulation parameters that influence adjuvanticity of liposomal vaccines include (A) vesicle size, (B) lamellarity, (C) membrane surface charge, (D) bilayer fluidity (as examples, cholesterol-rich liquid ordered and cholesterol-free liquid crystal phases are shown), (E) propensity to undergo lamellar-hexagonal bilayer phase transition, and (F) presence of immunosimulatory lipids.
