Table 1. Average volumes for each brain region compared across sex.

The average volume ± SEM for each brain region at each age is listed along with the statistical analysis for this data. We found a significant effect of sex at P0 within the CA3 of the hippocampus, as the volume of the female CA3 was significantly larger than the male CA3; and we found a significant effect of sex at P4 within the PCX, as the volume of the PCX was significantly larger in males than in females.

Age	Region	Avg. Volume (μm3) ± SEM	Avg. Volume (μm3) ± SEM	Statistics
		MALES	FEMALES
E17	PCX	17,185,000 ± 142,821	19,926,666 ± 1,895,364	t8= 1.16; p = 0.27
E17	AMY	30,286,666 ± 4,146,839	34,603,333 ± 1,489,884	t8= 0.98; p = 0.35
E17	CA1	4,608,333 ± 311,174	4,882,500 ± 335,556	t8= 0.60; p = 0.56
E17	CA3	3,125,692 ± 311,160	3,727,500 ± 455,112	t6= 1.09; p = 0.32
E17	DG	3,596,250 ± 473,120	4,889,304 ± 789,592	t6= 1.41; p = 0.21
E17	PVN	1,417,500 ± 355,354	778,750 ± 57,820	t6= -1.77; p = 0.13
P0	PCX	199,727,500 ± 15,796,814	180,955,834 ± 16,788,394	t6= -0.81; p = 0.45
P0	AMY	88,305,000 ± 9,252,067	91,700,000 ± 3,013,220	t6= 0.35; p = 0.74
P0	CA1	13,912,500 ± 581,200	19,232,500 ± 1,700,204	t6= 2.96; p = 0.02
P0	CA3	13,226,108 ± 1,164,681	17,762,500 ± 733,261	t6= 3.29; p = 0.02*
P0	DG	10,640,000 ± 1,806,205	13,396,250 ± 722,586	t6= 1.42; p = 0.21
P0	PVN	4,436,250 ± 551,250	5,783,750 ± 979,101	t6= 1.20; p = 0.28
P4	PCX	470,096,667 ± 20,828,640	283,360,000 ± 46,750,048	t6= -3.6; p = 0.02*
P4	AMY	156,248,750 ± 23,043,942	162,146,250 ± 24,951,106	t6= 0.17; p = 0.87
P4	CA1	54,845,000 ± 10,190,260	55,720,000 ± 11,098,762	t6= 0.06; p = 0.96
P4	CA3	49,201,250 ± 5,038,123	45,718,750 ± 7,196,683	t6= -0.40; p = 0.71
P4	DG	58,100,000 ± 9,494,681	57,540,000 ± 2,587,239	t6= -0.05; p = 0.95
P4	PVN	4,410,000 ± 533,678	5,013,750 ± 784,236	t6= 0.64; p = 0.55
P30	PCX	338,152,500 ± 22,458,741	330,455,412 ± 40,735,467	t6= -0.17; p = 0.87
P30	AMY	310,590,000 ± 52,838,994	301,096,250 ± 76,112,164	t6= -0.10; p = 0.92
P30	CA1	41,956,250 ± 4,099,992	35,673,750 ± 2,273,625	t6= -1.34; p = 0.23
P30	CA3	35,612,500 ± 3,570,757	47,710,831 ± 7,168,026	t6= 1.51; p = 0.18
P30	DG	59,351,250 ± 13,275,118	68,985,000 ± 10,304,464	t6= 0.57; p = 0.59
P30	PVN	3,202,500 ± 294,395	3,255,000 ± 705,375	t6= 0.07; p = 0.95
P60	PCX	315,953,750 ± 16,844,101	343,568,750 ± 12,659,927	t6= 1.31; p = 0.24
P60	AMY	217,017,500 ± 26,143,248	171,605,000 ± 13,181,245	t6= -1.55; p = 0.17
P60	CA1	62,868,750 ± 5,759,518	72,527,850 ± 5,590,957	t6= 1.20; p = 0.27
P60	CA3	63,560,000 ± 5,907,937	69,685,000 ± 5,869,730	t6= 0.74; p = 0.49
P60	DG	91,446,250 ± 11,536,003	75,993,750 ± 7,698,249	t6= -1.14; p = 0.31
P60	PVN	4,121,250 ± 135,836	3,788,750 ± 523,905	t6= -0.61; p = 0.56