Skip to main content
. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Cell Physiol. 2012 Aug;227(8):3119–3127. doi: 10.1002/jcp.23065

Figure 3. Neuronal survival after treatment with conditioned medium from agnoprotein-expressing CG4 cells.

Figure 3

A. Analysis of the effect of CXCL5/LIX on the survival of rat cortical neurons in the MTT assay. Rat cortical neurons were treated with CM and cell viability was evaluated after 16 hours. The order of samples is as follows: 1. neuronal culture medium; 2. CG4-Ol CM; 3. CG4-Ol GFP CM; 4. CG4-Ol GFP-Agno CM; 5. CG4-Ol GFP CM + neutralizing anti-LIX antibodies (3 µg/ml); 6. 1h pretreatment with rLIX (100 ng/ml) + CG4-Ol GFP-Agno CM; 7. CG4-Ol GFP CM + IgG1 (3µg/ml); 8. CG4-Ol GFP-Agno CM + BSA (100ng/ml). The relative cell viability (percent) for each sample was determined as the ratio of average absorbance for treated to that for untreated cells (sample 1). B. Effect of CXCL5/ LIX on the apoptosis of rat cortical neurons in the nexin assay. Flow cytometry with Annexin V-PE and Nexin 7-ADD. Rat cortical neurons were treated with CM and cell viability was evaluated after 16 hours and presented as percentage of double-positive cells. Sample order is the same as in panel A.