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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mol Oral Microbiol. 2011 Dec 7;27(2):70–82. doi: 10.1111/j.2041-1014.2011.00632.x

Figure 4. Detection of leukotoxin in the rmlC mutant strain overexpressing TolC.

Figure 4

(A). Immunoblot analysis. Equivalent amounts of outer membrane (OM) protein of the rmlC mutant (rmlC), the rmlC complemented strain (rmlC/rmlC+), and the TolC overexpression strain (rmlC/tolC+) were separated, transferred to PVDF, and probed with anti-LtxA antibody. (B). Quantification of leukotoxin (LtxA). The integrated intensity of each band in panel A, which represents the relative protein level, was quantified using the ImageJ program. The membrane-bound leukotoxin found in the rmlC/tolC+ strain remained the same as the rmlC mutant. The signal intensity of the rmlC mutant was arbitrarily set as 1.0. (ns: nonsignificant)