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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mol Oral Microbiol. 2011 Dec 7;27(2):70–82. doi: 10.1111/j.2041-1014.2011.00632.x

TABLE 1.

Strains and plasmids

Strains and plasmids Genotypes/Remarks Sources and references
A. actinomycetemcomitans
VT1169 a Rifr, Nalr derivative of SUNY465, serotype b (Mintz, 2004)
VT1169/pKM2/ltxP VT1169 transformed with plasmid pKM2/ltxP, which is referred as the “wild type” This study
aae mutant (aae::Kan) An isogenic aae (epithelial adhesin) mutant of SUNY465 (Rose et al., 2003)
rmlC (rmlC::pLOF/Sp) Rhamnose mutant, transposon pLOF/Sp inserted into rmlC(TDP-4-keto-6-deoxy-D-glucose 3, 5-epimerase) (Tang & Mintz, 2010)
rmlC/pKM2/ltxP rmlC mutant transformed with pKM2/ltxP This study
rmlC /rmlC+ rmlC mutant complemented with plasmid pKM2/ltxP/rmlC (Tang & Mintz, 2010
rmlC /tolC+ rmlC mutant transformed with plasmid pKM2/ltxP/tolC to over-express TolC This study
Plasmids
pLOF/Sp Tn10-based transposon vector; Apr, Spr (Mintz, 2004)
pKM2 A plasmid containing chloramphenicol acetyltransferase, which can be transformed into serotype b A. actinomycetemcomitans , Cmr (Gallant et al., 2008)
pKM2/ltxP pKM2 containing ~500-bp ltx_promoter, Cmr (Tang & Mintz, 2010)
pKM2/ltxP/rmlC pKM2 containing ~500-bp ltx promoter + rmlC sequence, Cmr (Tang & Mintz, 2010)
pKM2/ltxP/tolC pKM2 containing ~500-bp ltx promoter + tolC sequence, Cmr This study

Ap: ampicillin; Cm: chloramphenicol; Nal: nalidixic acid; Rif: rifampicin; Sp: spectinomycin