TABLE 2.

Primers and probes for quantitative real-time, reverse transcriptional PCR

Targets	Primers and probes	Sequence (5’–3’)	Amplicons (bp)
LktA	Forward primer	5’-GGACCTGTCGCAGGGTTAATT-3’	98
	Reverse primer	5’-AGCATTCTCGCACGATCAAA-3’
	Dual-labeled probe	5’-FAM-TCAGCTTGGCAATCAGCCCTTTGTC-BHQ-3’
16S rRNA*	Forward primer	5'-GAACCTTACCTACTCTTGACATCC-3'	122
	Reverse primer	5'-GGACTTAACCCAACATTTCACAAC-3'
	Dual-labeled probe	5'-FAM-CTGACGACAGCCATGCAGCACCTG-BHQ1-3'

^*Endogenous control

Reporter dye: 6-FAM; Quencher dye: BHQ-1