Table 1.
Target genes | Primer sequence | Size (bp) | Annealing temperature (°C) | |
GAPDH | Sense | 5'-CCACCCATGGCAAATTCCATGGCA-3' | 593 | 62 |
Antisense | 5'-AACAAAGCCTGGACAAAT-3' | |||
15-PGDH | Sense | 5'-GCTGGAGTGAATAATGAGA-3' | 285 | 55 |
Antisense | 5'-GCTGAGCGTGTGAATCCAACT-3' | |||
Survivin | Sense | 5'-GGCATGGGTGCCCCGAGGTT-3' | 320 | 58 |
Antisense | 5'-AGAGGCCTCAATCCATGGCA-3' | |||
BCL-2 | Sense | 5'-GGTGCCACCTGTGGTCCACCT-3' | 458 | 54 |
Antisense | 5'-CCTCACTTGTGGCCCAGATAGG-3' | |||
BAX | Sense | 5'-CTGACATGTTTTCTGACGGC-3' | 289 | 54 |
Antisense | 5'-TCAGCCCATCTTCTTCCAGA-3' | |||
BCL-XL | Sense | 5'-TTGGACAATGGACTGGTTG-3' | 765 | 54 |
Antisense | 5'-GTAGAGTGGATGGTCAGTG-3' | |||
BAK | Sense | 5'-TGAAAAATGGCTTCGGGGCAAGGC-3' | 642 | 54 |
Antisense | 5'-TCATGATTTGAAGAATCTTCGTACC-3' | |||
p53 | Sense | 5'-CCTTCCCAGAAAACCTACCA-3' | 371 | 59 |
Antisense | 5'-TCATAGGGCACCACCACACT-3' | |||
p21 | Sense | 5'-CAGGGGACAGCAGAGGAAGA-3' | 335 | 63 |
Antisense | 5'-GGGCGGCCAGGGTATGTAC-3' | |||
p27 | Sense | 5'-ATGTCAAACGTGCGAGTGTC-3' | 395 | 58 |
Antisense | 5'-TCTGTAGTAGAACTCGGGCAA-3' | |||
p16 | Sense | 5'-GGGCTCTCACAACTAGGAA-3' | 371 | 59 |
Antisense | 5'-CGGAGGAGGTGCTATTAACTC-3' |
15-PGDH: 15-hydroxyprostaglandin dehydrogenase.