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. 2012 Mar 14;18(10):1028–1037. doi: 10.3748/wjg.v18.i10.1028

Table 1.

Polymerase chain reaction primers

Target genes Primer sequence Size (bp) Annealing temperature (°C)
GAPDH Sense 5'-CCACCCATGGCAAATTCCATGGCA-3' 593 62
Antisense 5'-AACAAAGCCTGGACAAAT-3'
15-PGDH Sense 5'-GCTGGAGTGAATAATGAGA-3' 285 55
Antisense 5'-GCTGAGCGTGTGAATCCAACT-3'
Survivin Sense 5'-GGCATGGGTGCCCCGAGGTT-3' 320 58
Antisense 5'-AGAGGCCTCAATCCATGGCA-3'
BCL-2 Sense 5'-GGTGCCACCTGTGGTCCACCT-3' 458 54
Antisense 5'-CCTCACTTGTGGCCCAGATAGG-3'
BAX Sense 5'-CTGACATGTTTTCTGACGGC-3' 289 54
Antisense 5'-TCAGCCCATCTTCTTCCAGA-3'
BCL-XL Sense 5'-TTGGACAATGGACTGGTTG-3' 765 54
Antisense 5'-GTAGAGTGGATGGTCAGTG-3'
BAK Sense 5'-TGAAAAATGGCTTCGGGGCAAGGC-3' 642 54
Antisense 5'-TCATGATTTGAAGAATCTTCGTACC-3'
p53 Sense 5'-CCTTCCCAGAAAACCTACCA-3' 371 59
Antisense 5'-TCATAGGGCACCACCACACT-3'
p21 Sense 5'-CAGGGGACAGCAGAGGAAGA-3' 335 63
Antisense 5'-GGGCGGCCAGGGTATGTAC-3'
p27 Sense 5'-ATGTCAAACGTGCGAGTGTC-3' 395 58
Antisense 5'-TCTGTAGTAGAACTCGGGCAA-3'
p16 Sense 5'-GGGCTCTCACAACTAGGAA-3' 371 59
Antisense 5'-CGGAGGAGGTGCTATTAACTC-3'

15-PGDH: 15-hydroxyprostaglandin dehydrogenase.