Table 2.
Selective Reaction Monitoring (SRM) transitions monitored for detection of Ara h 1, Ara h 2 and Ara h 3.
| Peptides | Parention (m/z) (+) | Fragmention(s) (m/z) | CE a |
|---|---|---|---|
| Ara h 1 | |||
| VLLEENAGGEQEER | 786.9 (+2) | 989.5 (y9)/875.4 (y8)/804.4 (y7)/747.3 (y6)/561.3 (y4)/304.2 (y2) | 40 |
| DLAFPGSGEQVEK | 688.8 (+2) | 1077.5 (y10)/930.5 (y9)/833.4 (y8)/447.2 (b4)/300.2 (b3)/229.1 (b2) | 36 |
| Ara h 2 | |||
| CCNELNEFENNQR | 863.8 (+2) | 1050.5 (y8)/807.4 (y6)/660.3 (y5)/531.3 (y4) | 43 |
| NLPQQCGLR | 543.3 (+2) | 858.4 (y7)/761.4 (y6)/633.3 (y5)/200.1 (a2) | 29 |
| CDLEVESGGR | 561.2 (+2) | 846.4 (y8)/604.3 (y6)/505.2 (y5)/376.2 (y4) | 30 |
| CMCELQQIMENQSDR | 1006.9 (+2) | 1721.8 (y14)/1361.7 (y11)/1248.6 (y10)/992.5 (y8)/879.4 (y7)/748.3 (y6)/ 619.3 (y5)/292.1 (b2) | 49 |
| Ara h 3 | |||
| LNAQRPDNR | 361.9 (+3) | 1083.6 (ymax)/970.5 (y8)/856.4 (y7)/657.4 (y5)/228.1 (b2) | 19 |
| SPDIYNPQAGSLK | 695.4 (+2) | 1389.7 (ymax)/1302.7 (y12)/977.5 (y9)/814.5 (y8)/700.4 (y7)/475.3 (y5)/300.1 (b3) | 36 |
| AHVQVVDSNGNR | 432.5 (+3) | 749.5 (b7)/663.3 (y6)/535.4 (b5) | 23 |
a CE: collision energy. Dwell time (DT) for each transition was 0.05 s.