Table 1.
List of tryptic fragments from Conohyal-Cn1 identified with a glycosylation pattern by ESI-MS/MS. Residue B accounts for carboxyamidomethyl-Cys, (m) stands for the protonated molecular mass and (z) corresponds to the ion charge state.
| Fragment mass (Da) | m/z (z) | Peptide sequence | Sequence mass (Da) | Glycosylation | Glycosylation mass (Da) |
|---|---|---|---|---|---|
| 2,557.84 | 853.62 (3) | NVTQMMTDBSR | 1,341.45 | (HexNAc)2 (Hex)5 | 1,216.39 |
| 2,233.74 | 1,117.87 (2) | NVTQMMTDBSR | 1,341.45 | (HexNAc)2 (Hex)3 | 892.29 |
| 2,071.70 | 1,036.85 (2) | NVTQMMTDBSR | 1,341.46 | (HexNAc)2 (Hex)2 | 730.26 |
| 3,540.30 | 1,181.10 (3) | AIYSTNFGPMTIYQNESVK | 2,161.83 | (HexNAc)2 (Hex)6 | 1,378.47 |
| 3,378.12 | 1,127.04 (3) | AIYSTNFGPMTIYQNESVK | 2,161.73 | (HexNAc)2 (Hex)5 | 1,216.39 |
| 3,054.09 | 1,019.03 (3) | AIYSTNFGPMTIYQNESVK | 2,161.8 | (HexNAc)2 (Hex)3 | 892.29 |
| 2,892.14 | 965.05 (3) | AIYSTNFGPMTIYQNESVK | 2,161.88 | (HexNAc)2 (Hex)2 | 730.26 |
| 3,916.49 | 980.12 (4) | HRPENFTGLGVLDFETWR | 2,172.88 | (HexNAc)3 (Hex)7 | 1,743.61 |
| 3,551.27 | 888.81 (4) | HRPENFTGLGVLDFETWR | 2,172.8 | (HexNAc)2 (Hex)6 | 1,378.47 |
| 3,227.22 | 807.81 (4) | HRPENFTGLGVLDFETWR | 2,172.86 | (HexNAc)2 (Hex)4 | 1,054.36 |