Analysis of C. neoformans strain CAP67 supernatant fractions by SDS-PAGE. Crude (CR), flow-through (FT), and MP fractions were resolved by 12% SDS-PAGE and analyzed by (A) silver stain, (B) PAS, and (C) Con A–biotin blot. Migration of commercial molecular mass standards, expressed in kilodaltons, is indicated to the left of the gels. (Reproduced and adapted with permission from Mansour, M. K., Schlesinger, L.S., and Levitz, S. M. 2002. Optimal T cell responses to Cryptococcus neoformans mannoprotein are dependent on recognition of conjugated carbohydrates by mannose receptors. Journal of Immunology
168, 2872–2879.)