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. 2012 Mar 7;18(9):979–985. doi: 10.3748/wjg.v18.i9.979

Figure 1.

Figure 1

Interleukin-8 expression of gastric cancer cells. A: Real-time polymerase chain reaction determined the level of Interleukin-8 (IL-8) mRNA in gastric cancer (GC) cells. Expression levels were determined by the ΔΔCt method using glyceraldehyde-3-phosphate dehydrogenase as endogenous control. Histograms show fold change over the relative expression levels of IL-8 mRNA of control cells. Each bar represents the mean ± SD (aP < 0.05 vs MKN-45, bP < 0.05 vs KATO-III); B: IL-8 production in GC cells measured by enzyme-linked immunosorbent assay. Histograms show fold change in the relative expression levels of IL-8 protein of control cells. All experiments were repeated three times with similar results. Each bar represents the mean ± SD (aP < 0.05 vs MKN-45, bP < 0.05 vs KATO-III).