Effect of Interleukin-8 on NF-κB and Akt activities and adhesion molecules ICAM-1 and VCAM-1, and CD44 expression in gastric cancer cells. The protein expression levels of phospho-NF-κB-p65, NF-κB-p65, phospho-Akt, Akt, ICAM-1, VCAM-1, and CD44 were determined by Western blotting. All experiments were repeated three times with similar results.