Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The TaqMan assay, also known as the 5′ nuclease assay utilizes a third oligonucleotide labeled with a fluorophore (green) and quencher (gray), in which the fluorophore is quenched due to FRET conditions. During the reaction, the DNA polymerase (blue) degrades the probe, separating the fluorophore and quencher, allowing for fluorescence emission to occur.
