Figure 7.

Effects of PGE₂ and EP receptor agonists on the release of VEGF by primary adult lung fibroblasts. Primary adult lung fibroblasts were cultured until they reached 90% confluence in 12-well tissue culture plates, after which the medium was changed to SF-DMEM with PGE₂ or EP receptor agonists (all at 1 × 10⁻⁷ M). After 2 days, the monolayer culture medium was harvested and assayed for VEGF by ELISA. Vertical axis, level of VEGF release expressed as fold of control values. Horizontal axis, conditions. Control sample, without addition, is indicated by open circles. Each normal adult strain is indicated by a single type of symbol. *P < 0.05, compared with values of control sample.