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. 2012 Jan;2(1):106–127. doi: 10.1002/ece3.34

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© 2011 The Authors. Published by Blackwell Publishing Ltd.

This is an open access article under the terms of the Creative Commons Attribution Non Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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16S rDNA-based DGGE assay targeting type II MOB. DNA was extracted from soil cores sampled in May 2000 in four plots covering differences in flooding regime. Cores were subsectioned into three soil layers (0–5 cm, 5–10 cm, and 10–20 cm). Depicted are DGGE profiles as derived from soil directly coming from the field (in situ, left panel) and as obtained from DNA extracted from slurries at the end of methane oxidation assays (incubation, right panel). Bands are numbered and correspond to the sequences as presented in Table A5 as well as Figure 10.