Table A3.
ANOVA analyses of the effects of flooding plain elevation (i.e. site), depth in soil profile and season on initial- and induced methane oxidation activity, most probable numbers of MOB, abundance of MOB-specific PLFA in situ and in vitro, root biomass and soil moisture content. All variables were LN transformed to meet the assumptions for ANOVA analyses. For all samples n = 4. P values in bold are statistically significant (p < 0.05)
| Df | F | P | Nature of effect | |
|---|---|---|---|---|
| Initial CH4 oxidation | ||||
| Site (St) | 3 | 11.6803 | 0.000000 | + with flooding |
| Depth (Dp) | 2 | 4.501 | 0.013324 | − in deeper layers |
| Season (Sn) | 2 | 6.861 | 0.001583 | + in winter |
| St*Dp | 6 | 3.236 | 0.005870 | |
| St*Sn | 6 | 6.683 | 0.000005 | |
| Dp*Sn | 4 | 0.415 | 0.797660 | |
| St*Dp*Sn | 12 | 0.591 | 0.845338 | |
| Induced CH4 oxidation | ||||
| Site (St) | 3 | 21.15 | 0.000000 | + with flooding |
| Depth (Dp) | 2 | 3.98 | 0.021548 | − in deeper layers |
| Season (Sn) | 2 | 18.73 | 0.000000 | + in winter |
| St*Dp | 6 | 6.41 | 0.000009 | |
| St*Sn | 6 | 0.0930 | 0.000223 | |
| Dp*Sn | 4 | 0.76 | 0.553286 | |
| St*Dp*Sn | 12 | 1.31 | 0.224434 | |
| Most probable numbers | ||||
| Site (St) | 3 | 112.03 | 0.000000 | + with flooding |
| Depth (Dp) | 2 | 2.27 | 0.108008 | |
| Season (Sn) | 2 | 17.37 | 0.000000 | + winter |
| St*Dp | 6 | 1.30 | 0.264698 | |
| St*Sn | 6 | 11.86 | 0.000000 | |
| Dp*Sn | 4 | 0.34 | 0.853292 | |
| St*Dp*Sn | 12 | 0.45 | 0.938636 | |
| Root dry matter | ||||
| Site (St) | 3 | 23.8344 | 0.000000 | + with flooding |
| Depth (Dp) | 2 | 23.8649 | 0.000000 | − in deeper layers |
| Season (Sn) | 2 | 0.3203 | 0.726677 | |
| St*Dp | 6 | 5.1160 | 0.000126 | |
| St*Sn | 6 | 1.8691 | 0.000126 | |
| Dp*Sn | 4 | 1.7866 | 0.137343 | |
| St*Dp*Sn | 12 | 0.8761 | 0.573337 | |
| Moisture% | ||||
| Site (St) | 3 | 978.8 | 0.000000 | + with flooding |
| Depth (Dp) | 2 | 87.4 | 0.000000 | − in deeper layers |
| Season (Sn) | 2 | 36.5 | 0.000000 | + winter |
| St*Dp | 6 | 15.3 | 0.000000 | |
| St*Sn | 6 | 19.5 | 0.000000 | |
| Dp*Sn | 4 | 0.4 | 0.793398 | |
| St*Dp*Sn | 12 | 1.8 | 0.053910 | |
| C16:1ω5t in situ | ||||
| Site | 3 | 50.287 | 0.000000 | + with flooding |
| Depth | 2 | 12.123 | 0.000100 | − in deeper layers |
| Site*Depth | 6 | 2.245 | 0.061620 | |
| C16:1ω8c in situ | ||||
| Site | 3 | 128.4019 | 0.000000 | + with flooding |
| Depth | 2 | 20.36 | 0.000001 | − in deeper layers |
| Site*Depth | 6 | 7.3189 | 0.000040 | |
| C16:1ω5t in vitro | ||||
| Site | 3 | 32.769 | 0.000000 | + with flooding |
| Depth | 2 | 8.379 | 0.001738 | − in deeper layers |
| Site*Depth | 6 | 5.268 | 0.001380 | |
| C16:1ω8c in vitro | ||||
| Site | 3 | 22.6195 | 0.000000 | + with flooding |
| Depth | 2 | 4.6893 | 0.019095 | − in deeper layers |
| Site*Depth | 6 | 4.5852 | 0.003104 | |