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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: Cell. 2011 Nov 11;147(4):773–788. doi: 10.1016/j.cell.2011.08.054

Figure 3. Pc2 Methylation/Demethylation Controls Mitogenic Signal-Induced Cell Proliferation.

Figure 3

(A and B) Pc2 is essential for serum-induced growth control gene expression and cell proliferation. HeLa cells transfected with Pc2 siRNAs were serum-starved followed by restimulation and mRNA level of indicated genes (A) and cell proliferation (B) were analyzed, respectively. (C) Pc2K191 methylation is a key regulator for cell proliferation. HeLa cells transfected with Pc2 plasmids (wt or K191R mutant) were serum-starved followed by restimulation and cell proliferation were analyzed. (D and E) IMR-90 cells were serum-starved followed by restimulation and Pc2K191 methylation level (D) and cell proliferation (E) was analyzed by immunoblotting and flow cytometry, respectively. For B, C and E, cell proliferation was analyzed by monitoring the EDU incorporation into DNA synthesis in S-phase cells. Mean±SEM, *p<0.05 and **p<0.01.

See also Figure S3.