Table 1.

Primers and conditions for DNA methylation analysis.

Sequence ID	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Sequencing primer (5′ to 3′)	Sequence analyzed†
Gene-specific methylation analysis
iNOS	AATGAGAGTTGTTGGGAAGTGTTT	Biotin-CCACCAAACCCAACCAAACT	TAAAGGTATTTTTGTTTTAA	C/TGATTTTC/TGGGTTTTTTTTTATTTTG
IL-6	Biotin-TATTTTAGTTTTGAGAAAGGAGGTG	CAATACTCTAAAACCCAACAAAAAC	TCCTAATACAAACAACCCC	CG/AACCACACG/ACAAAAAC
Global methylation analysis
Alu	Biotin-TTTTTATTAAAAATATAAAAATT	CCCAAACTAAAATACAATAA	AATAACTAAAATTACAAAC	G/AC/TG/AC/TG/ACCACCA
LINE-1	TTTTGAGTTAGGTGTGGGATATA	Biotin-AAAATCAAAAAATTCCCTTTC	AGTTAGGTGTGGGATATAGT	TTC/TGTGGTGC/TGTC/TG

^†Nucleotides at which DNA methylation was measured are underlined.

LINE-1: Long interspersed nucleotide element 1.