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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: J Immunol. 2011 Nov 9;187(12):6310–6317. doi: 10.4049/jimmunol.1101812

Figure 3. Th-1 but not Th-2, Th-17 or Treg are capable of inducing DC tumoricidal activity.

Figure 3

Day 6 CD11c+ DCs were cultured for 48 hours with the supernatants from Th-1, Th-2, Th-17 and Treg cultures. DCs were then washed extensively and incubated for 48 hours with (A) 4T1 or (B) B16 tumor cells. KDCs activated with LPS (1 µg/ml) ([DC] LPS) were used as positive controls. Tumor cell survival was then determined. Mean +/− SD from triplicate cultures. (*p < 0.001). (C) Phenotype of DCs obtained after 48 hours of culture with the culture supernatant from Th-1, Th-2, Th-17 and Treg cultures. Cells were stained with the indicated Ab and analyzed by flow cytometry.