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. 2012 Feb 6;5:27. doi: 10.1186/1756-3305-5-27

Table 2.

Expression of AGAP003306 (OBP2) and AGAP0099629 (control) relative to expression of S7 in antennae of female and male A. gambiae in three biological replicates (experiments 1, 2 and 3).


Target Experiment Female Male Relative Expression

Cт Mean Cт SD ΔCт Mean Cт Mean Cт SD ΔCт Mean ΔΔCт RQ

1 35.55 0.105 36.51 0.257
Reference S7 2 33.31 0.335 35.04 0.125
3 33.57 0.565 32.05 0.188

1 29.77 0.208 -5.780 33.87 0.414 -2.634 -3.145 8.849
AGAP003306 2 29.77 0.168 -3.540 33.87 0.414 -1.170 -2.370 5.171
3 30.85 0.091 -2.721 33.99 0.309 1.941 -4.662 25.309

1 35.42 0.298 -0.130 36.06 0.353 -0.450 0.320 0.801
AGAP009629 2 35.55 0.105 2.239 36.56 0.390 1.518 0.721 0.607
3 36.46 0.478 2.894 34.95 0.686 2.894 0.000 1.000

= (cycle threshold) number of cycles required for the fluorescent signal to reach threshold

Cт Mean = Average Cт from triplicate values on same sample in same PCR reaction

Cт SD = Standard Deviation of triplicate Cт

ΔCт Mean = Cт MeanTarget- Cт MeanReference (target=AGAP003306 or AGAP009629; reference=S7)

ΔΔCт = ΔCт MeanFemale - ΔCт MeanMale

RQ = Relative Quantification = 2-(ΔΔCт)