Table 2.
Target | Experiment | Female | Male | Relative Expression | |||||
Cт Mean | Cт SD | ΔCт Mean | Cт Mean | Cт SD | ΔCт Mean | ΔΔCт | RQ | ||
1 | 35.55 | 0.105 | 36.51 | 0.257 | |||||
Reference S7 | 2 | 33.31 | 0.335 | 35.04 | 0.125 | ||||
3 | 33.57 | 0.565 | 32.05 | 0.188 | |||||
1 | 29.77 | 0.208 | -5.780 | 33.87 | 0.414 | -2.634 | -3.145 | 8.849 | |
AGAP003306 | 2 | 29.77 | 0.168 | -3.540 | 33.87 | 0.414 | -1.170 | -2.370 | 5.171 |
3 | 30.85 | 0.091 | -2.721 | 33.99 | 0.309 | 1.941 | -4.662 | 25.309 | |
1 | 35.42 | 0.298 | -0.130 | 36.06 | 0.353 | -0.450 | 0.320 | 0.801 | |
AGAP009629 | 2 | 35.55 | 0.105 | 2.239 | 36.56 | 0.390 | 1.518 | 0.721 | 0.607 |
3 | 36.46 | 0.478 | 2.894 | 34.95 | 0.686 | 2.894 | 0.000 | 1.000 |
Cт = (cycle threshold) number of cycles required for the fluorescent signal to reach threshold
Cт Mean = Average Cт from triplicate values on same sample in same PCR reaction
Cт SD = Standard Deviation of triplicate Cт
ΔCт Mean = Cт MeanTarget- Cт MeanReference (target=AGAP003306 or AGAP009629; reference=S7)
ΔΔCт = ΔCт MeanFemale - ΔCт MeanMale
RQ = Relative Quantification = 2-(ΔΔCт)