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. 2012 Feb 1;12:51. doi: 10.1186/1471-2407-12-51

Figure 1.

Figure 1

Expression of miR-133a in breast tissue specimens and cell lines. A. Strongly positive expression of miR-133a in tissues of breast benign disease. Magnification: ×200. B. Strongly positive expression of miR-133a in an adjacent normal breast tissue. Magnification: ×200. C. Weakly positive expression of miR-133a in an invasive ductal carcinoma. Magnification: ×200. D. Fresh tissue samples from patients with breast cancer or benign breast diseases were obtained from the surgery room. Total miRNA was extracted from the tissues and subjected to qRT-PCR analysis. E. Expression of miR-133a and FSCN1 in breast cancer cell lines: Breast cell lines were grown in monolayer for 3-5 days, and miRNA and total RNA was extracted and subjected to qRT-PCR analyses. F. Association of miR-133a expression with relapse-free survival of the patients with breast cancer.