Figure 2.

Different expression of α(1,6)fucosylated proteins in healthy and tumour colorectal tissues. (A) Representative Coomassie brilliant blue stained 1D-SDS-PAGE gel of the total protein fraction (TF) and the following chromatographic fractions obtained; FI (non-retained proteins), FII (unspecific retained proteins) and FIII [α(1,6)fucosylated proteins] and the corresponding lectin blot in a pair of healthy and tumour tissue. (B) Patern of bands obtained in the FIII fraction of a pair of healthy and tumour samples. Bands 5 and 8 were excised and submitted to mass spectrometry (MS) analysis.