Table 2.
Identification of α(1,6)fucosylated proteins by mass spectrometry
| Protein | Band n° | Mr (kDa) | NCBInr Swiss-Prot accession n° | Function | Score | Coverage rate (%) |
|---|---|---|---|---|---|---|
| Glucose regulated glycoprotein of 94 kDa | 5 | 92.7 | B4DHT9 | Stress response/chaperone | 743 | 20 |
| Polymeric immunoglobulins receptor | 5 | 84.4 | PO1833 | Immune response | 383 | 10 |
| Vascular adhesión protein 1 | 5 | 85.1 | Q16853 | Cell adhesion molecule/Immune response | 361 | 11 |
| Fibulin-1 C | 5 | 78.5 | P23142-4 | Extracellular matrix component/cell adhesion and migration | 339 | 12 |
| Platelet glycoprotein IIIa | 5 | 87.4 | PO5106 | Cell adhesion molecule/Immune response | 156 | 5 |
| IgG Fc binding protein | 8 | 59.6 | Q9Y6R7 | Cell adhesion molecule/Immune response | 1320 | 6 |
| Unknown protein product | 8 | 53.8 | Q6ZVX0 | unknown | 343 | 24 |
| Immunoglobulin G B12, heavy chain | 8 | 51.2 | gi|15825648 | Cell adhesion molecule/Immune response | 272 | 16 |
| Immunoglobulin α-1, constant region | 8 | 54.3 | P01876 | Cell adhesion molecule/Immune response | 248 | 14 |
Each protein score represents the sum of the score of each ion protein analyzed and this is, in turn considered numerically as -10 * log (p), where p is the probability that the matching was made randomly and coverage rate (%) represents the percentage of the total protein sequence that overlap with the peptide fragments.