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. 2012 Mar 8;8(3):e1002531. doi: 10.1371/journal.pgen.1002531

Figure 4. Candidate enhancers predicted by the classifier are active in FCs.

Figure 4

In situ hybridization of dve in wild-type (WT) embryos and embryos over-expressing Ras (Twi>Ras) in the mesoderm (A). Note the increased activity of dve in Twi>Ras embryos, indicative of a FC gene [22]. GFP driven by the classifier-predicted enhancers associated with the upstream sequences of slou (arrows in B) and slp1 (arrows in C). Slou protein (magenta) co-expresses with GFP (green) in slou-GFP embryos (B). Duf (magenta), which marks all FCs, co-expresses with slp1-GFP (green) (C). GFP (D) driven by the classifier-predicted intronic sequence associated with the dve gene co-expresses with Mef2 (D′) in myotubes at stage 15 in dve-GFP embryos.