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. 2012 Mar 8;8(3):e1002586. doi: 10.1371/journal.pgen.1002586

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Mirouze et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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With PsdpA-yfp (panel A) or with Pspo0A-yfp (panel B). Cells were segmented microscopically and the average pixel intensities in the CFP and YFP channels were recorded for each cell. The green boxes surround competence-expressing cells, with the lower limit of the boxes set from the threshold value (36) derived from Figure 8. These cells comprise 12.6% of the 11,238 cells measured for panel A and 14.2% of the 14,369 cells measured for panel B. The numbers of cells with CFP values in excess of the threshold were deposited into bins and displayed as a histogram, normalized to the total number of cells within each bin of YFP values. These data have been plotted on a histogram as a percent of total competent cells, with similar results (Figure S7).