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. 2012 Mar 8;7(3):e32910. doi: 10.1371/journal.pone.0032910

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Li, Padgett.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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All brains are positioned for a horizontal view. All pictures are maximum projections from multiple sections. Anti-Repo staining (red) is used to label glial cells. ombC-Gal4 expression is visualized by UAS-CD8-GFP (green). Glial cells located between white brackets (< >) correspond to the three layers of laminal glial cells: epithelial, marginal and medulla glia. Glial cells in the lamina are located between two arrows. Genotypes: (A, A′) UAS-CD8-GFP/+; ombC-Gal4/+; (B, B′) UAS-CD8-GFP/+; ombC-Gal4/UAS-ban; (C–D′) UAS-omb/UAS-CD8-GFP; ombC-Gal4/UAS-ban. (E–G) Histograms of green glia number in the optic lobe of the third instar larvae. (E) total green glia in the optic lobe; (F) the green glia in the lamina; (G) the green glia in the medulla. Scale bar: 50 µm.