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. 2012 Mar 8;7(3):e32674. doi: 10.1371/journal.pone.0032674

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Tiengwe et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Input (I) and eluate (E) samples from immunoprecipitations (IPs) using antibody against HA (anti-HA) are shown from procyclic T. brucei whole cell extracts of cells co-expressing TbORC1/CDC6-Myc (ORC-myc) and either Tb7980-HA (IP labelled ORC1/CDC6-7980) or Tb3120-HA (IP labelled ORC1/CDC6-3120); a control anti-HA IP is shown from cells expressing only Myc-tagged TbORC1/CDC6. Samples were separated by SDS-PAGE, transferred to a nylon membrane and probed with anti-HA antibody (upper panel) or with anti-Myc antibody (lower panel). Bands corresponding with immunoglobulin heavy chain (Ig), an anti-HA cross-reacting band (*) and with either HA-tagged Tb7980 or Tb3120 are indicated in the HA IP samples; size markers (kDa) are indicated.