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. 2012 Mar 8;7(3):e33101. doi: 10.1371/journal.pone.0033101

Figure 1. EMAP II overexpression delays cell proliferation.

Figure 1

Cell doubling, proliferation and progression through cell cycle were examined in clonal A549 cell populations overexpressing either EMAP II (pFEII-GFP) or GFP (pEGFP-N3). Western analysis of cell lysates using either EMAP II (A) or GFP (B) antibody confirmed expression. EMAP II overexpressing cells were found to have a marked delay in cell doubling (C, p<0.05), growth rate (D, p<0.001), and a delay in exiting the G2M phase of cell cycle as noted at 14 and 15 hours post synchronized cell release (*, E) as compared to the empty vector control (pEGFP-N3) (these experiments were performed a minimum of 4 times).