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. 2012 Mar 8;7(3):e33101. doi: 10.1371/journal.pone.0033101

Figure 4. EMAP II undergoes nuclear/cytoplasmic partitioning during cell cycle.

Figure 4

EMAP II distribution was assessed in chemically synchronized cell populations. Immunofluoresence indicated EMAP II expression is predominately nuclear in G0 (A,B) and S (C,D) phase, whereas in G2M (E/F) nuclear EMAP II is reduced. Western blot analysis (G) of isolated nuclear and cytoplasmic fractions indicates EMAP II expression is maintained in both compartments through out cell cycle (these experiments were performed 4 times). PSORT II analysis determined there were three classical types of nuclear localizing sequences (NLS): pat4, pat7, and a bipartite NLS (H). Proteins K and R composition predicts that 87% of EMAP II is subcompartmentalization to the nucleus EMAP II's (I). Magnification in A–F: 1000×.