Figure 5. EMAP II contains a Cdk1 binding site and immuno-precipitates with Cdk1 during S and G2M phases of cell cycle.

A Cdk1 binding domain, TPLH, was identified in EMAP II's N-terminal region at aa 84–87 (A). Immunoprecipitation using Cdk1 determined that EMAP II binds to Cdk1 during S and G2M phase of cell cycle (B) (these experiments were performed 4 times).