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. 2012 Mar 8;7(3):e33101. doi: 10.1371/journal.pone.0033101

Figure 6. Cdk1 phosphorylates EMAP II at amino acid 84, threonine.

Figure 6

Two potential Cdk1 phosphorylation sites were identified, aa 84 threonine, and serine at aa 232 (A). Using recombinant EMAP II protein isolates (B) in a Cdk1 assay, only the N-terminal and full-length EMAP II were phosphorylated by Cdk1 (C). Point mutation within the threonine (aa 84) to change the amino acid to either asparagine or alanine eliminated Cdk1 phosphorylation, thus confirming that the 84 aa threonine was the site of Cdk1 phosphorylation of EMAP II (D) (these experiments were performed 3 times).