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. 2012 Mar 8;7(3):e33101. doi: 10.1371/journal.pone.0033101

Figure 7. Phosphorylated EMAP II is predominately localized in the nuclear fraction.

Figure 7

A phosphorylated pThr84-EMAP II antibody was obtained (A) and phospo-EMAP II was examined in nuclear and cytoplasmic fractions during cell cycle. Phospho-EMAP II was found in the nuclear fraction G0-S phase of cell cycle with only the phosphorylated form found in the cytoplasm during G2M. Unphosphorylated EMAP II (capable of recognizing unphosphorylated and phosphorylated EMAP II) was confined to the cytoplasm as only a single band corresponding with the phosphorylated form was found in the nuclear isolates (B). Lamin B nuclear envelope confirmed nuclear isolate specificity (these experiments were performed a minimum of 4 times).