Figure 7. SCD1 modulated saturated FA-induced ROS levels in cardiac myocytes.

Cardiac myocytes were (A) infected with Ad-LacZ as a control or Ad-SCD1 at an MOI of 20 or (B) transfected with siGFP as a control or siSCD1 at 20 µM, and simultaneously treated with palmitic acid (PA, 250 µM) or stearic acid (SA, 250 µM). Intracellular ROS levels were detected with CM-H₂DCFDA. PA or SA-induced ROS levels in neonatal rat cardiac myocytes transduced with Ad-LacZ, and these ROS levels were reduced by Ad-SCD1 transduction. In contrast, knockdown of SCD1 by siRNA increased basal ROS levels, and enhanced PA or SA-induced ROS levels. Scale bar = 200 µm.