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. Author manuscript; available in PMC: 2012 Sep 8.
Published in final edited form as: Nature. 2012 Feb 19;483(7388):209–212. doi: 10.1038/nature10801

Figure 4. dpiezo and ppk function in parallel pathways.

Figure 4

(a) Mechanical nociception assay using a 45 mN von Frey filament with double null mutant of dpiezo and painless. Single knockout strains were used as controls and the wild type strain is w1118. n > 60. (b) Mechanical nociception assay on heterozygous larvae for dpiezo and/or pain. n (heterozygote dpiezo KO) = 74 from three trials, n (heterozygote painless1) = 169 from five trials, n (trans-heterozygote) = 166 from five trials. (c) Mechanical nociception assay with PPK and Piezo knockdown. ppk and/or dpiezo RNAi were driven by ppk-GAL4. n > 90. * p < 0.5, *** p < 0.001. n (wild type, w1118) = 66, n (UAS-ShiDN/+) = 75 and n (ppk-GAL4/+; UAS-ShiDN/+) = 73 with three trials. (d) Gentle touch sensitivity assay with ppk and dpiezo knockdown. Wild type is w1118. n > 90. (e) Thermal nociception assay using 45 °C probe with ppk and dpiezo knockdown. n > 75.