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. 1989 Oct;84(4):1124–1129. doi: 10.1172/JCI114275

Effects of insulin and exercise on muscle lipoprotein lipase activity in man and its relation to insulin action.

B Kiens 1, H Lithell 1, K J Mikines 1, E A Richter 1
PMCID: PMC329768  PMID: 2677048

Abstract

The effects of exercise and a physiological increase in plasma insulin concentration on muscle lipoprotein lipase activity (mLPLA), leg exchange of glucose, and serum lipoprotein levels were investigated in healthy young men. During euglycemic hyperinsulinemia (n = 7) at 44 mU.liter-1, m-LPLA in non-exercised muscle decreased from 30 +/- 7.4 mU.g-1 wet weight (w.w.) (mean +/- SE) to 19 +/- 3.3 (P less than 0.05). Furthermore, the decrease in m-LPLA correlated closely (r = 0.97, P less than 0.05) with the increase in leg glucose uptake. Moreover, basal m-LPLA correlated with the insulin-induced increase in leg glucose uptake (r = 0.93, P less than 0.05). In the control group (n = 6) in which saline was infused in place of insulin and glucose, m-LPLA in nonexercised muscle did not change with time. No change in m-LPLA was observed immediately after one-legged knee extension exercise, but 4 h after exercise m-LPLA was higher (P less than 0.05) in the exercised thigh (47 +/- 17.8 mU.g-1 w.w.) compared with the contralateral nonexercised thigh (29 +/- 6.3 mU.g-1 w.w.). This difference was not found 8 h after exercise. The triacylglycerol content of serum lipoproteins decreased during insulin infusion. It is concluded that in contrast to the effect on adipose tissue, physiological concentrations of insulin decrease m-LPLA in proportion to the effect of insulin on muscle glucose uptake, while muscle contractions cause a local, delayed, and transient increase in m-LPLA. Further-more, basal m-LPLA is an indicator of muscle insulin sensitivity.

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Selected References

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  1. Andersen P., Adams R. P., Sjøgaard G., Thorboe A., Saltin B. Dynamic knee extension as model for study of isolated exercising muscle in humans. J Appl Physiol (1985) 1985 Nov;59(5):1647–1653. doi: 10.1152/jappl.1985.59.5.1647. [DOI] [PubMed] [Google Scholar]
  2. Andersen P., Saltin B. Maximal perfusion of skeletal muscle in man. J Physiol. 1985 Sep;366:233–249. doi: 10.1113/jphysiol.1985.sp015794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arner P., Bolinder J., Engfeldt P., Lithell H. The relationship between the basal lipolytic and lipoprotein lipase activities in human adipose tissue. Int J Obes. 1983;7(2):167–172. [PubMed] [Google Scholar]
  4. Bartlett S. M., Gibbons G. F. Short- and longer-term regulation of very-low-density lipoprotein secretion by insulin, dexamethasone and lipogenic substrates in cultured hepatocytes. A biphasic effect of insulin. Biochem J. 1988 Jan 1;249(1):37–43. doi: 10.1042/bj2490037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bazelmans J., Nestel P. J., Nolan C. Insulin-induced glucose utilization influences triglyceride metabolism. Clin Sci (Lond) 1983 May;64(5):511–516. doi: 10.1042/cs0640511. [DOI] [PubMed] [Google Scholar]
  6. Christensen N. J., Vestergaard P., Sørensen T., Rafaelsen O. J. Cerebrospinal fluid adrenaline and noradrenaline in depressed patients. Acta Psychiatr Scand. 1980 Feb;61(2):178–182. doi: 10.1111/j.1600-0447.1980.tb00577.x. [DOI] [PubMed] [Google Scholar]
  7. DeFronzo R. A., Tobin J. D., Andres R. Glucose clamp technique: a method for quantifying insulin secretion and resistance. Am J Physiol. 1979 Sep;237(3):E214–E223. doi: 10.1152/ajpendo.1979.237.3.E214. [DOI] [PubMed] [Google Scholar]
  8. Essén B., Lindholm A., Thornton J. Histochemical properties of muscle fibres types and enzyme activities in skeletal muscles of Standardbred trotters of different ages. Equine Vet J. 1980 Oct;12(4):175–180. doi: 10.1111/j.2042-3306.1980.tb03420.x. [DOI] [PubMed] [Google Scholar]
  9. Górski J., Stankiewicz-Choroszucha B. The effect of hormones on lipoprotein lipase activity in skeletal muscles of the rat. Horm Metab Res. 1982 Apr;14(4):189–191. doi: 10.1055/s-2007-1018965. [DOI] [PubMed] [Google Scholar]
  10. Jacobs I., Lithell H., Karlsson J. Dietary effects on glycogen and lipoprotein lipase activity in skeletal muscle in man. Acta Physiol Scand. 1982 May;115(1):85–90. doi: 10.1111/j.1748-1716.1982.tb07048.x. [DOI] [PubMed] [Google Scholar]
  11. Kern P. A., Mandic A., Eckel R. H. Regulation of lipoprotein lipase by glucose in primary cultures of isolated human adipocytes. Relevance to hypertriglyceridemia of diabetes. Diabetes. 1987 Nov;36(11):1238–1245. doi: 10.2337/diab.36.11.1238. [DOI] [PubMed] [Google Scholar]
  12. Kern P. A., Marshall S., Eckel R. H. Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes. J Clin Invest. 1985 Jan;75(1):199–208. doi: 10.1172/JCI111675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kiens B., Lithell H. Lipoprotein metabolism influenced by training-induced changes in human skeletal muscle. J Clin Invest. 1989 Feb;83(2):558–564. doi: 10.1172/JCI113918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lillioja S., Young A. A., Culter C. L., Ivy J. L., Abbott W. G., Zawadzki J. K., Yki-Järvinen H., Christin L., Secomb T. W., Bogardus C. Skeletal muscle capillary density and fiber type are possible determinants of in vivo insulin resistance in man. J Clin Invest. 1987 Aug;80(2):415–424. doi: 10.1172/JCI113088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lithell H., Boberg J. Determination of lipoprotein-lipase activity in human skeletal muscle tissue. Biochim Biophys Acta. 1978 Jan 27;528(1):58–68. doi: 10.1016/0005-2760(78)90052-8. [DOI] [PubMed] [Google Scholar]
  16. Lithell H., Cedermark M., Fröberg J., Tesch P., Karlsson J. Increase of lipoprotein-lipase activity in skeletal muscle during heavy exercise. Relation to epinephrine excretion. Metabolism. 1981 Nov;30(11):1130–1134. doi: 10.1016/0026-0495(81)90059-7. [DOI] [PubMed] [Google Scholar]
  17. Lithell H., Hellsing K., Lundqvist G., Malmberg P. Lipoprotein-lipase activity of human skeletal-muscle and adipose tissue after intensive physical exercise. Acta Physiol Scand. 1979 Mar;105(3):312–315. doi: 10.1111/j.1748-1716.1979.tb06346.x. [DOI] [PubMed] [Google Scholar]
  18. Lithell H., Karlström B., Selinus I., Vessby B., Fellström B. Is muscle lipoprotein lipase inactivated by ordinary amounts of dietary carbohydrates? Hum Nutr Clin Nutr. 1985 Jul;39(4):289–295. [PubMed] [Google Scholar]
  19. Mangiapane E. H., Brindley D. N. Effects of dexamethasone and insulin on the synthesis of triacylglycerols and phosphatidylcholine and the secretion of very-low-density lipoproteins and lysophosphatidylcholine by monolayer cultures of rat hepatocytes. Biochem J. 1986 Jan 1;233(1):151–160. doi: 10.1042/bj2330151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nilsson-Ehle P., Schotz M. C. A stable, radioactive substrate emulsion for assay of lipoprotein lipase. J Lipid Res. 1976 Sep;17(5):536–541. [PubMed] [Google Scholar]
  21. Richter E. A., Mikines K. J., Galbo H., Kiens B. Effect of exercise on insulin action in human skeletal muscle. J Appl Physiol (1985) 1989 Feb;66(2):876–885. doi: 10.1152/jappl.1989.66.2.876. [DOI] [PubMed] [Google Scholar]
  22. Sadur C. N., Eckel R. H. Insulin stimulation of adipose tissue lipoprotein lipase. Use of the euglycemic clamp technique. J Clin Invest. 1982 May;69(5):1119–1125. doi: 10.1172/JCI110547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Saltiel A. R., Fox J. A., Sherline P., Cuatrecasas P. Insulin-stimulated hydrolysis of a novel glycolipid generates modulators of cAMP phosphodiesterase. Science. 1986 Aug 29;233(4767):967–972. doi: 10.1126/science.3016898. [DOI] [PubMed] [Google Scholar]
  24. Shimizu S., Inoue K., Tani Y., Yamada H. Enzymatic microdetermination of serum free fatty acids. Anal Biochem. 1979 Oct 1;98(2):341–345. doi: 10.1016/0003-2697(79)90151-9. [DOI] [PubMed] [Google Scholar]
  25. Standaert M. L., Farese R. V., Cooper D. R., Pollet R. J. Insulin-induced glycerolipid mediators and the stimulation of glucose transport in BC3H-1 myocytes. J Biol Chem. 1988 Jun 25;263(18):8696–8705. [PubMed] [Google Scholar]
  26. Strålfors P. Insulin stimulation of glucose uptake can be mediated by diacylglycerol in adipocytes. Nature. 1988 Oct 6;335(6190):554–556. doi: 10.1038/335554a0. [DOI] [PubMed] [Google Scholar]
  27. Vessby B., Boberg J., Gustafsson I. B., Karlström B., Lithell H., Ostlund-Linqvist A. M. Reduction of high density lipoprotein cholesterol and apoliproprotein A-I concentrations by a lipid-lowering diet. Atherosclerosis. 1980 Jan;35(1):21–27. doi: 10.1016/0021-9150(80)90024-6. [DOI] [PubMed] [Google Scholar]
  28. Yki-Järvinen H., Taskinen M. R., Koivisto V. A., Nikkilä E. A. Response of adipose tissue lipoprotein lipase activity and serum lipoproteins to acute hyperinsulinaemia in man. Diabetologia. 1984 Sep;27(3):364–369. doi: 10.1007/BF00304851. [DOI] [PubMed] [Google Scholar]

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