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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cancer. 2011 Dec 2;118(13):3433–3445. doi: 10.1002/cncr.26621

Figure 3. Inhibition of ROS diminishes HO-1 protein expression.

Figure 3

(A) Lower levels of intracellular peroxides in K562 cells treated with NAC or DPI. Histogram representation of flow cytometric data of DCF fluorescence, indicating intracellular peroxide levels, after treatment with 24 mM NAC (upper histogram) or 30 μM DPI (lower histogram). (B) ROS blockade using NAC or diphenylene iodonium (DPI; a NADPH oxidase inhibitor) causes a decrease in HO-1 protein expression. K562 cells were treated for 24 h with either diluent, 24 mM NAC or 30 μM DPI and HO-1 protein expression was monitored by Western blotting.