Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cancer. 2011 Dec 2;118(13):3433–3445. doi: 10.1002/cncr.26621

Figure 4. Chemical inhibition of the NADPH oxidase results in a greater decrease in intracellular ROS.

Figure 4

BaF3-p210 expressing cells were treated with 30 μM DPI for 4 hrs, 10 nM wortmannin for 4 hrs, or 1 μM rotenone for 1 hr. Histogram (A) and graphical (B) representation of intracellular peroxide levels measured by quantification of DCF fluorescence by flow cytometry. (C) Comparison of p47phox protein expression in total cell lysates from BaF3-vector and BaF3-p210 cells by Western blot. The * indicates a non-specific cross-reacting band. β-actin was used as a loading control and densitometry was performed using ImageJ software.