Table 2.
Sense and antisense primers used for quantitative real-time PCR (Sybr green)
| Gene | Accession No. | Sense primer (5'→3') | Antisense primer (5'→3') |
|---|---|---|---|
| ABCB1 (MDR1) | NM_000927.4 | ctggtgtttggagaaatgacag | aacctgaatgtaagcagcaacc |
| ABCB4 (MDR3) | NM_000443.3 | cttttccttgtcgctgctaaat | agttcagtggtgtcgttgatgt |
| ABCC2 (MRP2) | NM_000392.3 | ctcacttcagcgagaccg | ccagccagttcagggttt |
| ABCC3 (MRP3) | NM_003786.3 | aaaagcagacggcacgaca | gcaggcactgatgaggaagc |
| ABCB11 (BSEP) | NM_003742.2 | cattatccttccagaccagagg | tcttgctccactatcccaatct |
| SLC10A1 (NTCP) | NM_003049.3 | gatgaccacctgctccaccttc | tccctcccttgatgacatagcg |
| CYP7A1 | NM_000780.3 | acactttgtccacctttgatga | tcattgcttctgggttcctaat |
| SP1* | NM_138473.2 | gccgctcccaacttacagaa | cccatcaacggtctggaact |
| LRH-1(NR5A2) | NM_205860.1 | gcgtggaggaaggaataagt | gtcaggtcagagggcatagc |
| Actin | NM_001101.3 | ccacgaaactaccttcaactcc | gtgatctccttctgcatcctgt |
| GAPDH* | NM 002046.3 | ctttggtatcgtggaaggactc | gtagaggcagggatgatgttct |
*
Note: Primers were also used for semiquantitative RT-PCR as described previously (22).