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. Author manuscript; available in PMC: 2012 Sep 8.
Published in final edited form as: Nature. 2012 Feb 19;483(7388):176–181. doi: 10.1038/nature10812

Figure 2. Ruthenium red is a channel pore blocker of mpiezo1- but not dpiezo-induced currents.

Figure 2

(a) Representative traces of MA currents in mpiezo1-transfected cells evoked at holding potentials ranging from -80 to +80 mV before (left panel) and during perfusion of 30 μM of RR (right panel, red traces). (b) Average current-voltage relationship of MA currents in mpiezo1-transfected cells (n = 7 cells) before (black symbols) and during (red symbols) perfusion of 30 μM RR. Currents were normalized to the value of control current evoked at -80 mV for each individual cell. (c) Concentration-inhibition curve for RR on MA currents evoked at -80 mV in mpiezo1-transfected cells and fitted with a Boltzmann equation. Each data point is the mean ± s.e.m. of 3-13 observations. (d) Representative traces of piezo-dependent MA currents evoked at -80 mV in the presence of RR. (e) Blocking effect of RR on piezo-dependent MA currents evoked at -80 mV. Bars represent mean ± s.e.m. and the number of cells tested is shown above bars. *** P<0.001; ** P<0.01, unpaired t-test.