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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Photochem Photobiol. 2011 Dec 16;88(5):1191–1197. doi: 10.1111/j.1751-1097.2011.01038.x

Figure 1.

Figure 1

Fas and FADD distributions in gradient fractions of UVB-irradiated, cholesterol-depleted and cholesterol-enriched M624 cells. Total cell lysates were prepared from UVB-irradiated, cholesterol-depleted and cholesterol-enriched M624 cells and fractionated on an Optiprep continuous gradient (25-5%). Equal volumes of each gradient fraction was buffered and SDS-PAGE was performed. Proteins were electroblotted onto nitrocellulose membrane, the membrane was blocked, and the distributions of Fas and FADD were analyzed by immunoblot using the corresponding antibodies. Results are representative of three independent experiments.