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Representative immunohistochemistry images of 20× scanned slides. (Top row) (a–c) Colonic mucosa from a healthy control subject stained for serotonin (a), CD4 (b), and mast cell tryptase (c). (Bottom row) (d–f) Colonic mucosa from a patient with IBS-D stained for serotonin (d), CD4 (e), and mast cell tryptase (f). There are no qualitative differences based on immunohistochemically detected enterochromaffin, CD4T, or mast cells between healthy controls and IBS patients.
