Figure 3.

Comparison of motility between different cell types and different cargos. To examine the motility of various cargos in microtubule-disrupted cells, cells were first incubated with cargo (PV, transferrin (TFN), or Seneca Valley Virus (SVV)) for 10 min to allow internalization and were then treated with 60 μM nocodazole for 50 min to disrupt microtubules. More than 190,000 trajectories were analyzed. Error bars = SE. The comparison between PV and SVV were not carried out in HeLa S3 cells, but in H446 cells as the HeLa S3 cells cannot be infected by SVV. Survival function plots of the full speed distributions for the data shown here may be found in Fig. S9.