Figure 4.

Actin dependence of PV motility. (A) Effects of the actin perturbing drugs cytochalasin D (CCD, see also Movie S7), latrunculin A (LatA), latrunculin B (LatB), and jasplakinolide (Jas), and the myosin II inhibitor blebbistatin (Bleb) on the observed motility of PV in HeLa S3 cells. Survival function plots of the full speed distributions for the data shown here may be found in Fig. S11. (B) Inhibition of PV motility in H446 cells by treatment of CCD. (C) CCD inhibition of PV motility over time. HeLa S3 cells were pretreated for 50 min with nocodazole, infected with PV for 10 min, and then incubated with CCD. Movies were recorded at different time points (10–60 min) after CCD addition, indicating that significant inhibition of PV motility did not occur until at least 20 min after CCD incubation. Error bars = SE.