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. 2011 Dec 7;101(11):2798–2806. doi: 10.1016/j.bpj.2011.09.049

Figure 3.

Figure 3

Effect of NaCl on Rho regeneration and MetaII stability. (A) Rho regeneration in the presence of NaCl. Rho in 100 mM HEPES, pH 7.4, containing 0.02% DM, with a 1.5 molar excess of 11-cis-retinal, was preincubated with or without 3.5 M NaCl and then illuminated for 30 s. The plot shows the time course of Rho regeneration by monitoring the absorbance increase at 500 nm at room temperature. Values are the mean ± SE (n = 3). (B) MetaII stability was determined by monitoring Trp fluorescence at λ330nm to measure retinal release upon Schiff-base hydrolysis. Rho in 100 mM HEPES, pH 7.4, containing 0.05% DM, in the absence (−) or in the presence of 3.5 M NaCl (⋅⋅⋅), was illuminated for 30 s and Trp fluorescence was measured by fluorescence spectroscopy at 20°C. Experiments were performed in triplicate.