Figure 3.
Single-channel recordings of CaV1.2 channels modulated by CaVβ1a and CaVβ2b subunits and their mixtures. (A) Exemplary single-channel Ba2+ current traces of CaV1.2 channels after transient coexpression of CaVβ1a and CaVβ2b subunit as indicated. (Top row) Pulse protocol (150-ms pulses applied every 600 ms). (Middle) Ten representative consecutive traces are shown for each CaVβ subunit combination. (Bottom rows) Respective single-channel average currents (from at least 180 traces recorded per experiment). (B) Single-channel open probability fluctuates on a minute timescale. Diaries of single-channel open probability (lower panels of each graphs with noisy lines) were automatically split into temporal clusters with statistically significantly different mean open probability values (upper panels of each graph).