Figure 6.

Double-sided imaging of retina whole-mount preparations combined with local light-transmission. (a) The x-z view of a confocal stack of fluorescent images of retinal tissue stained with Mitotracker green, acquired using a 40×/1.2 W objective. Shown is a maximum y projection across seven pixels (corresponding to 2.4 μm) around the line indicated in panel b. The endfeet (top) and processes of the Müller cells are clearly visible. (Green triangles) Positions at which the laser beam was focused onto the tissue; (white line) z position of the image in panel b. (b) Confocal x-y image of the same tissue shown in panel a, acquired with a 10×/0.3 objective. This image was used to position the laser beam (positions indicated by green circles). The transmission images corresponding to the two larger spots are shown in panels c and d. (c and d) Transmission images acquired with the upright imaging unit while the laser focus was either placed in the periphery at Müller cell endfoot (c) or on top of a Müller cell process (d). The scale is identical to the scale used for panels a and b; both images show the same field of view. (e and f) Transmission images shown in panels c and d (green) superimposed with an overview image (red) acquired with the same camera during wide-field transmission-mode illumination of the retina's inner surface.