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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Nature. 2012 Feb 22;483(7387):96–99. doi: 10.1038/nature10852

Figure 2. Klf15 regulates KChIP2 expression in the heart.

Figure 2

(a) Kv4.2 mRNA expression exhibits diurnal rhythm in WT mice, but in Klf15-null hearts the rhythm is abnormal with reduced expression at ZT6 (ZT- Zeitgeber Time), and increased expression at ZT22 (n=4 per time point per group). (b) KChIP2 mRNA expression exhibits exhibit no rhythmic variation in Klf15-deficient mice, with significant reduction at all time points (n=4 per time point per group). (c) KChIP2 protein expression exhibits no variation across 12 hours in Klf15-null hearts. (d, e) Klf15-Tg mice hearts express higher levels of KChIP2 mRNA and protein. (f) Chromatin immunoprecipitation with Flag antibody illustrating enrichment of Flag-KLF15 on the KChIP2 promoter (n=3 per group). Data presented as mean ± SEM, *p<0.05.