Figure 6.

Inhibition of IKK activity restores the deregulated differentiation of Rassf2-deficient osteoclasts and osteoblasts. (A–D) BMMs (A, B) and osteoblast precursors (C, D) from WT or Rassf2^−/− mice were infected with a retroviral vector encoding Flag-tagged IKK-DN or with the empty vector, and were next stimulated with RANKL (A, B) or osteogenic medium (C, D). Cell lysates prepared at the indicated times were subjected to IB analysis with the indicated antibodies (A, C), and either the number of TRAP⁺ MNCs was determined after 4 days (B) or calcium deposition was measured after 12 days (D). Quantitative data are mean values±s.d. (n=4, ^*P<0.01). (E) Model for RASSF2-mediated inhibition of NF-κB signalling during osteoclast and osteoblast differentiation.