Figure 2.
Rp58 KO mice showed aberrant expression of Id mRNA. (A) Real-time PCR analysis of all four Id mRNAs in WT and mutant cerebral cortices at E18.5. Gapdh mRNA expression was used for normalization (*P<0.05, **P<0.01; t-test). Data represent the mean±s.d. (n=3). (B–I) Representative in situ hybridization images showing the four Id mRNAs in coronal brain sections from E18.5 WT (B–E) and mutant mice (F–I). All four Id mRNAs were ectopically expressed around the mutant SVZ (F–I) (indicated with star) (n=3). The dotted line in (C) indicates the edge of the VZ. Scale bars: 250 μm.